Pfizer doing SARS-COV2 Gain of Function

Torej vprašanje:
Ali kje obstaja objava/naloga/raziskava (recimo temu kakorkoli že hočemo) o izolaciji neznanega virusa iz vzorca (ne omejujem se na kovid; katerikoli vzorec in katerikoli neznani virus) z jasnim opisom metodologije in uporabljenih materialov, VKLJUČNO z uporabo kontrolnega vzorca, v katerem preverjeno ni virusov (recimo fiziološka raztopina)? Kontrolni vzorec bi moral biti uporabljen po enakem postopku in na enakem materialu kot vzorec, ki predvidoma vsebuje virus(e). Temu je kontrola v osnovi namenjena.

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Differential centrifugation is a classic and clever technique used to separate components in a mixture based on their size and density. When it comes to isolating viruses from cell debris, here’s how it works step by step:

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Principle Behind Differential Centrifugation​

• It relies on sedimentation rates: larger and denser particles settle faster under centrifugal force.
• By spinning the sample at increasing speeds, different components pellet out at different stages.

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Step-by-Step Process to Separate Viruses from Cell Debris​

1. Cell Lysis
   • The infected cells are broken open (lysed) to release viruses and cellular contents into a solution.
2. Low-Speed Centrifugation (~500–1,000 × g)
   • This removes large debris like nuclei and unbroken cells.
   • These form a pellet at the bottom, while smaller particles (including viruses) remain in the supernatant.
3. Medium-Speed Centrifugation (~10,000 × g)
   • This step pellets organelles like mitochondria and lysosomes.
   • Again, viruses stay in the supernatant.
4. High-Speed Centrifugation (~100,000 × g or more)
   • Now the viruses are pelleted because they’re smaller and require higher force to sediment.
   • The supernatant is discarded, and the virus-rich pellet is collected.

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Why It Works for Viruses​

• Viruses are smaller than most cellular components, so they remain suspended until the final high-speed spin.
• This method is crude but effective for separating viruses from bulk cell debris.

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Bonus Tip: Density Gradient Centrifugation​

If you need higher purity, you can follow up with density gradient centrifugation (e.g., using sucrose or cesium chloride). This separates particles based on density, not just size.

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Uporabnik dax je napisal:

KSZ Evo ga

Nova različica covida, že v Evropi: Prvi simptom precej neobičajen

Nova različica koronavirusa, znana kot Stratus ali XFG, se hitro širi po Veliki Britaniji in Irskem.

mariborinfo.com

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Uporabnik AndY1 je napisal:

Pi 5 letih Youtube daje nazaj accounte in video posnetke, ki so jih z casa Covida cenzurirali:

"This is about you guys. This is about our viewers and listeners."

V YouTubu lahko uživate v videoposnetkih in glasbi, ki so vam všeč, nalagate izvirno vsebino ter delite vse s prijatelji, družino in svetom.

youtube.com

Ocitno je, po toči, tudi YT spoznal, da so bile 'teorije zarote' resnicne.

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